Journal of ecology and environmental sciences eiss2330 piss2322 cultivation of microalga botryococcus braunii using red nile tilapia effluent medium for biogas production unpaprom y1, intasaen o1, yongphet p2, ramaraj r2,3 1program in biotechnology, faculty of science, maejo university, sansai, chiang mai50290, thailand. Repetitive nondestructive milking of hydrocarbons from. This study focuses on extracting oil from the microalgae matrix rather than the cells, eliminating the need for an excessive electric field to. The microalga botryococcus braunii is widely regarded as a potential. Sachs a,1 a department of earth, atmospheric and planetary sciences, building e34166, massachusetts institute of technology, 77 massachusetts avenue, cambridge, ma 029, usa. Botryococcus braunii species has gained lots of interest among the scientific community and biofuel industries due to its ability to synthesize and accumulate huge amount of lipids. These hydrocarbons can be extracted insitu without compromising cellular integrity, removing the need for dewatering and harvesting, providing. Botryococcus braunii is a microalga with an extracellular hydrocarbon matrix, which makes it an appropriate green energy source. Since uvvis spectrophotometric optical density od is the most commonlyused technique for estimating cell concentration in liquid culture. Stress responses of the oilproducing green microalga botryococcus. Results this study focuses on extracting oil from the microalgae matrix rather than the cells, eliminating the need for an excessive electric field to create electropermeabilization. For agar cultures, strains are transferred to the surface of fresh agar slants in 20 mm diamter glass screwcap tubes in preparation for shipment.
Latest researches on botryococcus braunii 41 botryococcus is a genus of green algae. Treatment of grey water using hydrocarbon producing. Appears as colonies of cells irregularly arranges cells in a folded mucilage. Other advantages of botryococcus braunii culture botryococcus braunii absorb high amount of carbondi oxide to maintain its growth rate. The chemotaxonomic criteria allow us to unequivocally characterize the organism collected from lake shira as botryococcus braunii, race a. Thats why high carbondioxide concentration can increase its growth rate. Botryococcus braunii in response to several stress inducers such as nacl. Botryococcus braunii for biodiesel production request pdf. Liquid hydrocarbon production potential of a novel strain of. The growth, lipid and hydrocarbon production of botryococcus braunii with attached cultivation. Botryococcus braunii is a fresh water green microalgae which synthesizes and stores hydrocarbons in a colonial extracellular matrix.
The micro algae culture botryococcus braunii was obtained from algae depot, usa and was maintained in chu medium. A new mechanism for efficient hydrocarbon electroextraction from. Transcriptomic analysis of a moderately growing subisolate. Botryococcus braunii, a green colonial microalga, is an unusually rich renewable source of hydrocarbons and other chemicals.
However a number of issues hamper its commercial development including, low growth rates and biomass production, low hydrocarbon yield, problems associated with scaling up of production, harvesting of biomass, and recovery. Pdf cultivation of botryococcus braunii strain in relation of its use. Botryococcus braunii as a bioreactor for the production of. Below is the link to the electronic supplementary material. Biomarker evidence for the cooccurrence of three races a. University of groningen potency of botryococcus braunii. Botryococcus braunii are unicellular, planktonic, ovalshaped microalgae.
Botryococcus braunii can use to treat waste water because it uptake dissolve phosphorus from water. The green alga botryococcus braunii is widely recognized as a potential carbonneutral source of hydrocarbons and biofuels. Pdf as tornabene1,2 has already discussed in this and in an earlier volume, many microorganisms photosynthetic or not are capable of producing. Production of biofuel by using micro algae botryococcus braunii. Pdf the production of hydrocarbons by botryococcus braunii. The green alga botryococcus braunii is regarded as a potential source of renewable fuel due to its high lipid and hydrocarbon contents. This article represents a first record of the presence of b. High biofuel production of botryococcus braunii using. Squalene and botryococcene are branchedchain, triterpene compounds that arise from the headtohead condensation of two molecules of farnesyl diphosphate to yield 1. Botryococcus medium directions for 1 l total prepare 940 ml of bristol medium. Colony organization in the green alga botryococcus braunii race. Catalytic conversion of botryococcus braunii oil to dropin.
Oct 17, 2017 production of biodiesel from botryococcus braunii 2. The microalga botryococcus braunii is known for its potential as a source of renewable energy by 1producing hydrocarbon fuels. A comprehensive and systematic metabolic characterization of the botryococcus braunii race a strain ccap 8072 was conducted within. Latest researches on botryococcus braunii oilgae club. Many researches had demonstrated that the biodiesel produced using the oil. Hi folks we have a new website, please check it out. One of their interests is how culture conditions affect the alga growth and the hydrocarbon production in order to find the optimal. The effect of media composition on the growth of b. Our experiments show that the addition of citrate to a culture can increase cell numbers by up to 20 times compared with the control in the first week. Botryococcus braunii is difficult to cultivate and has a limited amount of substantive scaleup and productivity assessments with conventionally suspended cultivation systems, such as open pond or. A novel alphaproteobacterial ectosymbiont promotes the growth. The extracellular matrix is a complex, three component system that all works together to produce the external hydrocarbons as well as protects each cell 14.
Data were recorded in softmax and exported into txt files. Biomarker evidence for the cooccurrence of three races a, b. Application of nacl for cultivation of isolated botryococcus braunii strains 1rashmi tyagi, 2tanisha manchanda, 3avdesh bhardawaj and 4r. Optimization of growth and hydrocarbon production from a. Microalgae generally contain less than 5% of dry weight as hydrocarbons, with the exception of the green alga, botryococcus braunii, in which contents of up to 61% of dry weight as hydrocarbons have been reported metzger et al. Potency of botryococcus braunii cultivated on palm oil mill.
Production of biofuel by using micro algae botryococcus. Bajpai 1,2,3,4department of applied sciences, itm university, sector 23 a, gurgaon, haryana, india. Media in category botryococcusthe following 3 files are in this category, out of 3 total. However, their extreme slow growth rate has impeded its application for renewable. Pdf the growth, lipid and hydrocarbon production of. Botryococcus braunii has an outstanding ability to produce lipid. Botryococcus braunii occupies a variety of aquatic habitats. Statistical optimization of culture media for growth and. The splash project will develop a new biobased industrial platform using microalgae as a renewable raw material for the sustainable production and recovery of hydrocarbons and exopolysaccharides from the species botryococcus braunii and further conversion to renewable polymers.
Recent understanding that specific algae have high hydrocarbon production potential has attracted considerable attention. The growth, lipid and hydrocarbon production of botryococcus. However, the slow growth rate damaged its feasibility for biofuel production. Liquid hydrocarbon production potential of a novel strain. Colonies held together by a lipid biofilm matrix can be found in tropical gold or oligotrophic lakes and estuaries, and will bloom when in the presence of elevated levels of inorganic dissolved phosphorus. Application of nacl for cultivation of isolated botryococcus braunii strains 229 2. The botryococcus braunii strain was studied by using. Produced from straight vegetable oil animal fats waste cooking oil. Botryococcus braunii is a green, colonial microalga that can produce up to 75 % of its dry weight as liquid hydrocarbons that can be converted to a sustainable biofuel. Lightharvesting complexes of botryococcus braunii springerlink. Growth curve for botryococcus braunii grown in the modified bold 3n medium. These hydrocarbons can be extracted insitu without compromising cellular integrity, removing the need for dewatering and harvesting, providing a unique opportunity to simplify the microalgae.
This thesis describes how using a heterotrophyrecoveryautotrophy route called the green cycle for the cultivation of botryococcus braunii results in high biofuel production. C 40 monoaromatic lycopane derivatives are indicative of b. The colonial microalga botryococcus braunii has been brought to peoples attention for its conspicuous ability to accumulate a variety of lipids including hydrocarbons. As part of the effort to combat the spread of covid19, the university of texas at austin has implemented a mandatory, campuswide reduction of laboratory activities to essential operations. Botryococcus braunii, microalgae, bubble column photo bioreactor, biofuels, saccharomyces cerevisiae, soxhlet apparatus. Lipid and hydrocarbon compositions of a collection strain and a wild.
A new mechanism for efficient hydrocarbon electroextraction. Cultivation of microalga botryococcus braunii using red. Variations in the density of botryococcus braunii at the 4 sampling sites and its mean density in paoay lake over the study time from may to september 2006. Botryococcus braunii, fuel conversion system, catalytic reaction, dropin fuel, cetane number oil extracted from b. Botryococcus braunii is considered a promising source of nonoxygenated liquid biofuels. Large variation in colony structure exists, yet its implications and influence of oil distribution and diffusion dynamics are not known and could not be answered due to lack of suitable in vivo methods. Biodiesel biodiesel is an alternative fuel similar to conventional or fossil diesel. In this study, a novel method of attached cultivation was introduced to incubate b.
Botryococcus braunii is an oleaginous green algae with the distinctive property of accumulating high quantities of hydrocarbons per dry weight in its colonies. Botryococcus braunii is a green colonial microalga that is widespread in freshwater, brackish lakes, reservoirs and ponds wake and hillen, 1980, 1981. Botryococcus braunii is a greencolonial alga containing significant quantities of hydrocarbons with a composition similar to that of heavy oil and has attracted a lot of academic and industrial researchers. The compounds initially present in treated grey water like nitrogen and phosphorus is reduced considerably and.
Botryococcus braunii is well known from all continents, but it has been sparsely recorded from africa compared to other continents find. Haematococcus pluvialis and botryococcus braunii are cultured commercially and their productions are established in different parts of the world. Full text botryococcus braunii as a bioreactor for the production of. Harvesting and preextraction processing of the algal biomass are among the major challenges for commercial sustainability of algaebased biofuel production. Borowitzka, in microalgae in health and disease prevention, 2018. Assessing botryococcus braunii hydrocarbon production. Also od can be used as a timely and indirect measurement of the increase in microalgal biomass in the cultivation.
Botryococcus braunii is a green algae that often forms extensive blooms and which, under appropriate conditions, can synthesize and secrete hydrocarbons that accumulate up to 86% of the alga dry weight. Borowitzka, in single cell oils second edition, 2010. Publishers pdf, also known as version of record publication date. Pdf botryococcus braunii, a new elicitor for secondary. Catalytic conversion of botryococcus braunii oil to drop. Hydrocarbons can constitute up to 75% of the dry mass of b. Aug 28, 2015 the colonial microalga botryococcus braunii has been brought to peoples attention for its conspicuous ability to accumulate a variety of lipids including hydrocarbons. Improving botryococcus braunii cultivation as a source of. Microalgae are sunlight driven cell factories that convert carbondioxide to potential biofuels. The highest contents, however, are known only from field samples, and the. Botryococcus braunii an overview sciencedirect topics. Statistical optimization of growth media was performed to faster growth and to increase lipid concentration. First record of botryococcus braunii kutzing from namibia.
An energyefficient and costeffective method of in situ recovery of the hydrocarbonrich oil from the harvest aqueous culture of b. Application of nacl for cultivation of isolated botryococcus. Botryococcus braunii, a new elicitor for secondary metabolite production in capsicum frutescens. A tiny green alga, botryococcus braunii is found worldwide, but most notably in oil and coal shale deposits. The botryococcus braunii strain was studied by using two media. Therefore, it is essential to conduct a comprehensive study about microalgae. Biofuel, botryococcus braunii, kinetic model, palm oil mill effluent, turbidity. The raw data have been supplied as supplementary files. Botryococcus braunii is a colonial green alga whose cells associate via a.
The fresh inocula are grown in a diurnal light cycle for at least 3 days until a macroscopically visible lawn appears. A novel alphaproteobacterial ectosymbiont promotes the. Until now, no studies have been performed on the effects of light quality on b. In the present investigation the antibacterial properties of different solvent extracts of these three micro algae were evaluated. A and b races are known to accumulate high level of lipids. Towards the commercialization of microalgal production. Botryococcus braunii is a green, pyramidshaped planktonic microalga that is of potentially great importance in the field of biotechnology. Botryococcus braunii is a green microalgae known for its ability to. The individuals live in a biofilm community that helps contribute to their external hydrocarbon production 3. Abstract one of the possible sources of renewable biofuel is the colonial green.
Botryococcus braunii is a colonyforming green alga that accumulates large amounts of liquid hydrocarbons within the colony. High cell growth and biofuel production of botryococcus braunii. The enzymes that catalyze their formation have attracted considerable interest from the medical field as potential drug targets and the renewable energy sector for metabolic engineering. Jan 04, 2018 botryococcus braunii bb is a green, pyramidshaped planktonic microalga that is of potential importance in the field of biotechnology. Cells spherical or oval, chloroplast netlike with a single pyrenoid. The effects of light quality on the morphology and. C 27,c 29,c 31 alkadienes and trienes were present in the pyrolysate of an elastic organic substance from the periphery of lake balkash, and derived from b. Fluorescence microscopy of botryococcus braunii colony.
793 437 698 787 361 1259 560 547 562 1590 284 213 278 344 522 245 877 376 979 1260 43 1407 1454 348 289 1389 457 947 1082 538 1563 112 856 852 1290 1215 525 501 681 1250 762 621 12